Laboratory Protocols

 

B.Tech. Lab Protocols

          BT 290: Biomolecular Analysis Laboratory

Experiment #

Title

1

Estimation of Protein

2

Estimation of Carbohydrate

3

Determination of Peroxide Value of lipid

4

Estimation of Ascorbic Acid

5

Estimation of Nucleic acid

6

Determination of melting temperature (Tm) of DNA

7

Partial purification of alkaline phosphate from potato and desalting of purified fraction

8

Electrophoresis of Protein (SDS-PAGE)

9

Activity staining and Isozyme analysis

10

Enzyme Kinetics

11

Protein Unfolding

12

Protein structure using structural coordinates procured from Protein Data Bank

        BT 330: Biochemical Engineering Laboratory

Experiment #

Title

1

Determination of biomass concentration by dry weight method

2

Determination of specific substrate utilization rate

3

Determination of specific growth rate

4

Growth kinetics at various substrate concentration

5

Deactivation kinetics of soluble enzyme

6

Batch operation in a bioreactor to study bacterial growth kinetics

7

Determination of KLa

8

Fed-batch operation to maintain exponential growth phase

                      


M.Tech. Lab Protocols

                        BT 510: Analytical Biotechnology Laboratory   
      

Experiment #

Title

1

Estimation of Carbohydrate

2

Estimation of DNA by Diphenylamine reagent

3

Analysis of DNA by Gel Electrophoresis

4

Determination of melting temperature (Tm) of DNA

5

Protein Extraction & Protein Estimation by Bradford reagent

6

Electrophoresis of Protein (SDS-PAGE)

7

Protein Unfolding

8

Biolistic Gun

9

HPLC

10

SEM

11

Confocal Microscopy

12

AFM
 
 

                    BT 520:  Applied Biology and Bio-engineering Laboratory
 

Experiment #

Title

1

ASEPTIC CULTURE TRANSFER TECHNIQUE

2

BACTERIAL GROWTH CURVE and SPECIFIC GROWTH RATE

3

BIOINFORMATICS TOOLS FOR SEQUENCE ANALYSIS, DESIGN OF PRIMERS AND IDENTIFICATION OF RESTRICTION SITES
 

4

PREPARATION OF COMPETENT CELLS

5

TRANSFORMATION OF COMPETENT CELLS WITH PLASMID DNA

6

ISOLATION OF PLASMID DNA

7

RESTRICTION ANALYSIS OF PLASMID DNA

8

PROTEIN EXPRESSION ANALYSIS by SDS-PAGE

9

Agrobacterium tumefaciens-mediated plant transformation and transient reporter gene expression assay
 
 



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