Gait analysis is an important criterion, nowadays, to diagnose various medical conditions of any individual. Moreover, the gender based disparities in a gait cycle can be witnessed in the social world. In this work, a Kinect-Labview based experimental setup is used to detect and estimate the gait kinematic parameters of ten healthy participants (5 male: 22.2 ± 2.14 years; 5 female: 21.8 ± 2.14 years) in sagittal plane. Primarily, hip, knee and ankle joint angles of right lower limb are evaluated for both gender groups. Thereafter, to draw a clear state of comparison between the gender groups, two sample t-test based statistical investigations are carried out for five prominent gait events at a significance level of 5%. From statistical results, significant gender based gait differences are found which might be useful in the early assessment of gait abnormalities.