Department of Biosciences and Bioengineering
Indian Institute of Technology Guwahati



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Department of Biosciences and Bioengineeeiring
Indian Institute of Technology Guwahati
Guwahati 781039

(0361) 258-2250
Fax: (0361) 258-2249

Rajaram Swaminathan, Ph.D.

Department of Biosciences and Bioengineering
Indian Institute of Technology Guwahati
Guwahati 781 039, Assam, India

Email: rsw [AT]
Tel: +91 361 2582248, 2582246
Fax: +91 361 2690762

Research Interests

A. Protein Structure Function and Dynamics

B. Consequences of Crowding inside living cells

C. BIG data analysis of the Proteomes

ORCID ID:  0000-0003-1294-8379



Doctor of Philosophy, 1996, Tata Institute of Fundamental Research, Mumbai (Mumbai University).

Master of Science (Biotechnology), 1990, Indian Institute of Technology, Bombay.

Bachelor of Science (Chemistry), 1988, A. M. Jain College, Chennai (Madras University).


Head, Biotechnology Department, IIT Guwahati from inception till March 2006

2002 onwards Faculty, Biotechnology (later Biosci. Bioengg.) Department, IIT Guwahati

1999-2002 Faculty, Chemistry Department, IIT Guwahati

1998-1999 Research Associate, National Centre for Ultrafast Processes, Chennai

1995-1998 Postdoctoral Fellow, Verkman Lab, CVRI, University of California, San Francisco, USA

Sponsored Projects

Title: Investigating the role of protein dynamics on the function of few disordered proteins
Funding source: Biotech Consortium of India Ltd.
Status: Completed

Title: Single molecule fluorescence investigations on the mechanism of lysozyme aggregation and RNA helicase activity
Funding source: DBT
Status: Completed

Title: Protein aggregation: Early molecular events, mechanisms and inhibition 
Funding source: DST
Status: Completed

Conjugating luminescent quantum dots to proteins: Consequences on protein function and development of sensitive assays.
Funding source: Council for Scientific and Industrial Research, New Delhi.
Status: Completed

Title: Tracking the growth of soluble protein aggregates in real time using fluorescence and
subsequent manoeuvres to inhibit their growth.
Funding source: Council for Scientific and Industrial Research, New Delhi.
Status: Completed

Title: Protein Folding: Looking for residual structures in denatured proteins
Funding source: Ministry of Human Resources and Development under the Research and
Development scheme
Status: Completed

Title: Effect of macromolecular obstacles on the kinetics of a chemiluminescent reaction.
Funding source: Department of Science and Technology, New Delhi.
Status: Completed 


Journal Publications

Ansari, Mohd. Z., A. Kumar, D. Ahari, A. Priyadarshi. P. Lolla, R. Bhandari and R. Swaminathan (2018) Protein charge transfer absorption spectra: an intrinsic probe to monitor structural and oligomeric transitions in proteins. Faraday Discussions (Photoinduced processes in Nucleic Acids and Proteins) DOI: 10.1039/c7fd00194k

Prasad, S., I. Mandal, S. Singh, A. Paul, B. Mandal, R. Venkatramani and R. Swaminathan (2017) Near UV-Visible electronic absorption originating from charged amino acids in a monomeric protein. Chem. Sci. 8, 5416-5433.

Somaiah C, A. Kumar, D. Mawrie, A. Sharma, S. D. Patil, J. Bhattacharyya, R. Swaminathan, B. G. Jaganathan (2015) Collagen Promotes Higher Adhesion, Survival and Proliferation of Mesenchymal Stem Cells. PLoS ONE 10(12): e0145068. doi:10.1371/journal.pone.0145068

Thokchom, A. K., R. Swaminathan and A. Singh (2014) Fluid Flow and Particle Dynamics Inside an Evaporating Droplet Containing Live Bacteria Displaying Chemotaxis. Langmuir 30,12144-12153

Iyer, A., A. Chandra and R. Swaminathan (2014) Hydrolytic enzymes conjugated to quantum dots mostly retain whole catalytic activity. BiochimBiophys. Acta 1840, 2935–2943

Ravi, V. K., M. Goel, H. C. Kotamarthi, S. R. K. Ainavarapu and R. Swaminathan (2014) Preventing Disulfide Bond Formation Weakens Non-covalent Forces Among Lysozyme Aggregates. PLoS One 9(2): e87012 doi 10.1371/journal.pone.0087012

Ravi, V. K., T. Swain, N. Chandra and R. Swaminathan (2014) On the characterization of intermediates in the isodesmic aggregation pathway of hen lysozyme at alkaline pH. PLoS ONE 9(1): e87256 doi 10.1371/journal.pone.0087256

Prasad, S. and R. Swaminathan (2013) Measuring the diffusion of fluorescent dye or protein inside living cells. Curr. Sci. 105, 1549-1561.

Mattaparthi Venkata Satish Kumar and Rajaram Swaminathan (2010) A novel approach to segregate and identify functional loop regions in
protein structures using their Ramachandran maps. Proteins: Struct. Func. Bioinformatics 78, 900-916.

Kumar, S, V. K. Ravi and Rajaram Swaminathan (2009) Suppression of Lysozyme Aggregation at Alkaline pH by tri-N-acetylchitotriose, Biochim.
Biophys. Acta. 1794, 913-920.

Kumar, S., Atul K. Singh, G. Krishnamoorthy and R. Swaminathan (2008) Thioflavin T displays enhanced fluorescence selectively inside anionic micelles and mammalian cells, J. Fluoresc. 18, 1199-1205.

Kumar, S., Ravi, V. K. and R. Swaminathan (2008) How do surfactants and DTT, affect the size, dynamics, activity and growth of soluble lysozyme aggregates?, Biochem. J. 415, 275-288.

Agrawal, M., S. B. Santra, Rajat Anand and R. Swaminathan (2008) Effect of macromolecular crowding on the rate of diffusion-limited enzymatic reaction, Pramana-J. Phys. 71, 359-368.

Dash, N., Chipem, F. A. S., Swaminathan, R. and Krishnamoorthy, G. (2008) Hydrogen bond induced twisted intramolecular charge transfer in 2-(4'-N,N-dimethylaminophenyl)imidazo [4,5-b]pyridine, Chem. Phys. Lett. 460, 119-124.

Kumar, S. and R. Swaminathan. Employing the fluorescence anisotropy and quenching kinetics of tryptophan to hunt for residual structures in denatured proteins, J. Chem. Sci., 119(2), 141-145 (2007) [Special issue on Fluorescence].

Homchaudhuri, L., Sarma, N. and R. Swaminathan. Effect of crowding by dextrans and Ficolls on the rate of alkaline phosphatase catalysed hydrolysis: A size dependent investigation., Biopolymers 83, 477-486 (2006).

Homchaudhuri, L., Kumar, S. and R. Swaminathan. Slow aggregation of lysozyme in alkaline pH monitored in real time employing the fluorescence anisotropy of covalently labelled dansyl probe., FEBS Letters, 580, 2097-2101 (2006).

Homchaudhuri, L. and R. Swaminathan. Near Ultraviolet Absorption Arising from Lysine Residues in Close Proximity: A Probe to Monitor Protein Unfolding and Aggregation in Lysine Rich Proteins, Bull. Chem. Soc. Japan, 77, 765-769 (2004).

Homchaudhuri, L. and R. Swaminathan Novel absorption and fluorescence characteristics of l-Lysine. Chemistry Letters 2001, 844-845 (2001).

Partikian, A., B. P. Olveczky, R. Swaminathan, Y. Li, and A. S. Verkman (1998). Rapid diffusion of green fluorescent protein in the mitochondrial matrix. J. Cell Biol. 140:821-829.

Swaminathan, R., C. P. Hoang and A. S. Verkman (1997). Photobleaching recovery and anisotropy decay of green fluorescent protein GFP-S65T in solution and cells: cytoplasmic viscosity probed by GFP translational and rotational diffusion. Biophys. J. 72:1900-1907.

Swaminathan, R., S. Bicknese, N. Periasamy and A. S. Verkman (1996). Cytoplasmic viscosity near cell plasma membrane: translational diffusion of a small fluorescent solute measured by total internal reflection-fluorescence photobleaching recovery. Biophys. J. 71:1140-1151.

Swaminathan, R., U. Nath, J. B. Udgaonkar, N. Periasamy and G. Krishnamoorthy (1996). Motional dynamics of a buried tryptophan reveals the presence of partially structured forms during denaturation of barstar. Biochemistry 35:9150-9157.

Swaminathan, R. and N. Periasamy (1996). Analysis of fluorescence decay by the maximum entropy method: influence of noise and analysis parameters on the width of the distribution of lifetimes. Proc. Indian Acad. Sci. (Chem. Sci.) 108:39-49.

Swaminathan, R., G. Krishnamoorthy and N. Periasamy (1994). Similarity of fluorescence lifetime distributions in single tryptophan proteins in the random coil state. Biophys. J. 67:2013-2023.

Swaminathan, R., N. Periasamy, J. B. Udgaonkar and G. Krishnamoorthy (1994). Molten globule-like conformation of barstar: a study by fluorescence dynamics. J. Phys. Chem. 98:9270-9278.

Book Chapters

Chhabra G, N. Chandra, R. Swaminathan (2017) Osmolytes: Key players in regulating protein aggregation in Cellular Osmolytes, L. Rajendrakumar Singh and T. A. Das (eds.), Springer Nature Singapore Pte Ltd. 2017. DOI 10.1007/978-981-10-3707-8_5

Swaminathan, R., V. K. Ravi, S. Kumar, M. V. S. Kumar and N. Chandra (2011) Lysozyme: A model protein for amyloid research. In Adv. Protein Chem. Struct. Biol. Vol. 84 R. M. Donev (editor), Academic Press, 2011, pp. 63-111. ISBN: 978-0-12-386483-3

Please click here for details on the citations of the publications above.


Courses taught recently: Biochemistry; Biophysics;

Elective Course offered in July 2018: Fluorescence Techniques in Biotechnology

Students & Staff

Ph.D. students

Ms. Alka Singh

Mr. Alark Shripad Kulkarni (with Prof. H. B. Nemade)

Mr. Ekramul Alom

Mr. Anurag Priyadarshi

Ms. S. Sanjana (with Prof. L. Rangan)

Mr. Amrendra Kumar

Mr. Dileep Ahari (with Prof. L. Rangan)

Mr. Mohd. Ziauddin Ansari

Ms. Shrutidhara Biswas

Ms. Saumya Prasad (Completed 2017)

Ms. Tulsi Swain (Completed 2018)

Mr. Nividh Chandra (Thesis submitted)

Mr. Vijay Kumar Ravi (Completed 2011)

Mr. M. Venkata Satish Kumar (Completed 2010)

Mr. Biswaranjan Meher (Completed 2009)

Mr. Satish Kumar (Completed 2009)

Ms. Lopamudra Homchaudhuri (Completed 2006)

M.Tech students

Mr. Subhajit Karmakar

Ms. Garima Chhabra

Ms. Bhanupriya Chouhan and Mr. Alark S. Kulkarni

Ms. Tasneem Ali

Mr. Ashish A. Patel

Mr. Jaspal Garg and Mr. Anil Chandra

Mr. Aditya Iyer and Mr. Kapil Dev Singh

Mr. C. Narendra Reddy

B.Tech Project students

Ameye Samant

Girnar Goyal (2017)

Duggirala B Reddy and Avasarala R Shilpa (2015)

Sanjana Gupta (2014)

Arushi Varshney (2012)

Mohit Goel and Bhaskar Ganesh Chennuri (2011)

Abhinav Shukla (completed 2010)

K. Akshay (completed 2010)

Ramesh Nadh (completed 2009)

I. Vikram Kumar Goud (completed 2008)

Raunak Jain (completed 2008)

Hari Singhal (completed 2008)

Ms. Purkanti Ramya (completed 2007)

Mr. Manish Agarwal (completed 2006)
Mr. Arun Atmanathan (completed 2006)

M.Sc Project student (CHEMISTRY DEPT.)

Ms. Navanita Sarma (completed 2002)

Indian Institute of Technology Guwahati 2013 Department of Biosciences and Bioengineering
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