B. Tech Biosciences and Bioengineering

BT 312 Analytical Biotechnology Laboratory 0-0-6-6

Syllabus: Theory, operation and handling of instruments to be used in this lab course, identification and determination of concentration of amino acid using thin layer chromatography, Determination of oligomeric status of the given protein by gel filtration chromatography, monitor equilibrium unfolding of a protein from the tryptophan fluorescence or Far UV-circular dichroism or enzymatic assay, determination of protein concentration by Bradford method, immuno-labeling and visualization of marker protein inside cells, screening of antibodies in radial immuno-diffusion assay, determination of concentration of antibody by ELISA, preparation of nanoparticle to detect bio-analyte, demonstration of flow cytometry with experimental sample, demonstration of real-time PCR with experimental sample, basics of microscopy with visualization of mammalian cells (unstained and stained sample), demonstration of SEM with experimental sample and demonstration of AFM with experimental sample.

Texts:

1. R. Boyer, Modern Experimental Biochemistry, 3^rd Edition, Pearson Education (Singapore) Pvt. Ltd., 2001.

2. R. L. Switzer and L. F. Garrity, Experimental Biochemistry, 3^rd Edition, W. H. Freeman, 1999.

3. J. Sambrook and D. W. Russell, Molecular Cloning: A Laboratory Manual, 3^rd Edition, Cold Spring Harbor Laboratory Press, 2001.

4. R. D. Goldman, J. R. Swedlow and D. L. Spector, Live Cell Imaging: A Laboratory Manual, 2^nd Edition, Cold Spring.Harbor Laboratory Press, 2009.

References:

1. K. Wilson and J. Walker, Principles and Techniques of Practical Biochemistry, 5^th Edition, Cambridge University Press, 2002.

2. A. J. Ninfa and D. P. Ballou, Fundamental Laboratory Approaches for Biochemistry and Biotechnology, 2^nd Edition, Wiley,1998.