B. Tech Biosciences and Bioengineering

BT 311 Biochemical Engineering Laboratory 0-0-6-6

Syllabus: Overview: understanding the different components of a bioreactor and general overview of laboratory theory, operation and handling of instruments to be used in this labotratory course; Production of hydrolytic enzymes (cellulase/hemicellulase) in shake flask; Determination of activity of hydrolytic enzyme; Free v/s immobilized enzyme kinetics; Pretreatment and enzymatic hydrolysis of lignocellulosic biomass; Estimation of mass transfer coefficient and mixing time in a bioreactor; Batch cultivation of S. cerevisiae in bioreactor; Fed-batch cultivation of S. cerevisiae in bioreactor; Downstream processing for ethanol recovery by distillation and quantification of distillation efficiency.

Texts:

1. O. Levenspiel, Chemical Reaction Engineering, 3^rd Edition, John Wiley & Sons, 1999.

2. J. E. Bailey and D. F. Ollis. Biochemical Engineering Fundamentals, 2^nd Edition, New York, McGraw Hill, 1986.

3. H. W. Blanch and D. S. Clark, Biochemical Engineering, Marcel Dekker, 1996.

References:

1. H. J. Rehm and G. Reed., Biotechnology - A Multi-volume Comprehensive Treatise, Volume 3, 2^nd Edition, Wiley VCH, 1993.

2. M. Moo-Young, Comprehensive Biotechnology, Volume 2, 2^nd Edition, Pergamon Press, 2004.