B. Tech Biosciences and Bioengineering

BT 303 Biochemical Engineering 2-1-0-6

Syllabus: Introduction to the field: definitions and concept; The kinetics of enzyme catalyzed reactions: enzyme-substrate complex and enzyme action, simple enzyme kinetics with one or two substrates, determination of elementary-step rate constant, patterns of substrates concentration dependence, modulation and regulation of enzyme activity, inhibition kinetics, enzyme deactivation, enzyme reaction in heterogeneous systems; Applied enzyme catalysis: application of hydrolytic enzymes, other applications of enzymes in solution, immobilized-enzyme technology, immobilized enzyme kinetics; Metabolic stoichiometry and energetics: thermodynamic principles, metabolic reaction coupling ATP and NAD, carbon catabolism, respiration, photosynthesis, biosynthesis, transport across cell membrane, metabolic organization and regulation, end products metabolism, stoichiometry of cell growth and product formation; Kinetics of substrate utilization, product formation and biomass production in cell cultures: kinetics measurements, kinetics of balanced growth, transient growth kinetics, product formation kinetics; Gas-Liquid mass transfer and heat transfer in bioprocess systems: gas-liquid mass transfer in cellular systems, determination of oxygen transfer rates, overall kLa estimates and power requirements for sparged and agitated vessel, factors affecting kLa, non-newtonian fluids, heat transfer, sterilization; Design and analysis of biological reactors: batch, fed-batch and continuous (CSTR and plug flow) operation modes, continuous with cell recycle, immobilized biocatalysis, multiphase bioreactors (packed bed, bubble column, air lift and membrane bioreactor), anaerobic fermentation technology, animal and plant cell reactor technology; Instrumentation and control: physical, chemical and biosensors, online sensors, computers and interfaces; Bioprocess scale up and economics.

Texts:

1. J. E. Bailey and D. F. Ollis, Biochemical Engineering Fundamentals, 2^nd Edition, New York, McGraw-Hill, 1986.

2. P. M. Doran, Bioprocess Engineering Principles, 2^nd Edition Elsevier Science & Technology Books, 2013.

3. H. W. Blanch and D. S. Clark, Biochemical Engineering, 2^nd Edition, CRC Press, Marcel, Dekker Inc., 1996.

References:

1. P.F. Stanbury and A. Whitaker, Principles of Fermentation Technology, 3^rd Edition, Pergamon Press, Elsevier, 2016.

2. B. Mattiason, Immobilized Cells and Organelles, Volume I, CRC Press, Florida, 2018.

3. M. L. Shuler, F. Kargi, Bioprocess Engineering: Basic Concepts, 2^nd Edition, Prentice Hall, 2002.