BT 211	Basic Biotechnology Laboratory	0-0-6-6
Pre-requisites: Nil Syllabus Theory, Operation and handling of instruments to be used in the lab course; Aseptic techniques for microbial culture; isolation of pure microbial culture and quantification of viable cells; Gram staining technique; preparation of chemically competent E.coli cells; Transformation of E.coli; small scale isolation of Recombinant plasmid from E.coli; analysis of the recombinant plasmid using Restriction enzymes; RNA isolation; Polymerase Chain Reaction; Estimation of DNA in solution; Estimation of protein in solution; Estimation of carbohydrate; Enzyme linked Immuno assay; Gel electrophoresis of protein; study of enzyme kinetics. Text Books 1. R. Boyer, Modern Experimental Biochemistry, 3rd Ed., Pearson Education (Singapore) Pvt. Ltd., 2001 2. R. L. Switzer and L. F. Garrity, Experimental Biochemistry, 3rd Ed., W. H. Freeman, 1999 3. S. J. Karcher, Molecular Biology: A Project Approach, Academic Press, 2001. References 1. J. Sambrook, D. W. Russell and J. Sambrook, Molecular Cloning, A laboratory Manual, Cold Spring Harbor Laboratory, USA, 1999. 2. J. G. Chirikjian, Biotechnology: Theory and Techniques (Genetic Engineering, Mutagenesis and Separation Technology), Jones & Bartlett Publishers, U.K., 1995. 3. H. Jones and John M. Walker, Plant Gene Transfer and Expression Protocols: Methods in Molecular Biology, 49, Humana Press, 1996. 4. J.G.Chirikjian, Biotechnology: Theory and Techniques (Plant Biotechnology, Animal Cell Culture and Immunobiotechnology), Jones & Bartlett Publishers, U.K., 1996. 5. D. M. Glover and B. D Hames, DNA Cloning II, IRL Press, 1995. 6. K. Wilson and J. Walker (ed.), Practical Biochemistry, Principles and Techniques, Cambridge University Press, 1995.